Antioxidant effects of aqueous fruit extract of Ziziphus jujuba on ethanol-induced oxidative stress in the rat testes

The aim of the present study was to evaluate the antioxidant effects of aqueous fruit extract of Ziziphus jujuba on ethanol-induced oxidative stress and to examine its protective effects on sperm motility and plasma membrane integrity of male Wistar rats. Adult male rats [n=28] were divided into four equal groups: the first group served as untreated controls, the second group of rats was given ethanol [4 g/kg], and group 3 received aqueous fruit extract of Z. jujuba [200 mg/kg]. The final group of rats were supplemented by oral ingestion of ethanol [4 g/kg], which was preceded 60 min earlier by an oral administration of Z. jujuba extract [200 mg/kg]. Fruit extract of Z. jujuba could prevent the reduction of concentration, motility and plasma membrane integrity of the sperm in treated rats with Z. jujuba extract plus ethanol [P<0.05]. Glutathione peroxidase and superoxide dismutase activities significantly increased in the animals ingested with Z. jujuba extract prior to ethanol compared to the ethanol group. Decrease of thiobarbituric acid reactive substances [TBARS] level was statistically significant in the animals that ingested the fruit extract of Z. jujuba prior to ethanol compared to the ethanol group [P<0.05]. Our findings suggest that aqueous fruit extract of Z. jujuba possesses beneficial effects on ethanol-induced sperm toxicity, subsequently enhancing sperm motility and plasma membrane integrity

Antibacterial activity and chemical composition of essential oils from four medicinal plants of lorestan, Iran

There is an increasing report of bacterial resistance to antibiotics in the world. Also side effects of chemical food preservatives suspected to be carcinogenic have drawn attention towards application of herbal medicine and natural substitutions. In this study, antibacterial activities of essential oils from four medicinal plants and chemical compositions of essential oils from Thymus [T.] eriocalyx and T. persicus of Lorestan were examined. Flowers and leaves from T. persicus, T. eriocalyx, Crocus sativus and Gundelia tolurntortel L. were collected freshly. Essential oils were prepared in Clevenger apparatus and analyzed by GC/MS. Antibacterial activities were examined by disk diffusion and broth microdillution technique, using standard [ATCC] gram positive and negative bacteria and standard [Muller Hinton agar and broth or Isosensitest agar] media in order to determine Minimum Inhibitory [MIC] or Bactericidal [MBC] concentration. Twenty seven compounds in essential oils from T. Eriocalyx and fifteen in T. persicus were identified. The major compounds were Thymol and Crvacrol; which constitute%66 and% 7.5 respectively in essential oils from T. erocalyx and%10 and%25 in T. persicus. Strong antibacterial activities were observed against both gram positive and negative bacteria including S. aureous and P. aeroginosa by T. eriocalyx and T. persicus. Essential oils from T. persicus produced MIC =MBC= 10 /LLg /ml against P. aeroginosa which is significant. Essential oils of leaves from Gundela tourntorel and Crocus sativus showed antibacterial activities against some gram positive bacteria. Significant antibacterial activities were observed in species examined from Thymus essential oils. Given the fact that T. eriocalyx is rich in thymol but low in carvacrol, application of this oils in medicine and food preservatives would avoid inflammatory effect of carvacrol and provide good prospects for further application of this popular plant